A simple and selective LC method is described for the determination of Ribociclib dosage forms. Chromatographicseparation was achieved on a c8 column using mobile phase consisting of a mixture of Sodium Phosphate Buffer (pH3.0): Acetonitrile (70:30) with detection of 284 nm. Linearity was observed in the range 50-150 μg /ml for Ribociclib(r2 =0.9926) for drugs estimated by the proposed methods was in good agreement with the label claim. Ribociclibwas found to be simple, precise, accurate and high resolution and shorter retention time makes this method moreacceptable and cost effective and it can be effectively applied for routine analysis in research institutions, qualitycontrol department in industries, approved testing laboratories, bio-pharmaceutical and bio-equivalence studies.Key Words: Ribociclib, chromatographic separation, high resolution, shorter retention, bioequivalence

A simple and selective LC method is described for the determination of Bilastine and Montelukast in tablet dosageforms. Chromatographic separation was achieved on a Waters AcquityC18 (50mm x2.1 mm ID) 1.8μm using mobilephase consisting of a mixture of 55 volumes of mixed Phosphate Buffer pH 3.5: Acetonitrile(75:25) %v/v withdetection of 265nm. Linearity was observed in the range 20-60 μg/ml for Bilastine(r2 =0.9995) and 10-30 μg/mlforMontelukast(r2 =0.9997) for the amount of drugs estimated by the proposed methods was in good agreement withthe label claim. From the above experimental results and parameters it was concluded that, this newly developedmethod for the simultaneous estimation Bilastine and montelukastwas found to be simple, precise, accurate and highresolution and shorter retention time makes this method more acceptable and cost effective and it can be effectivelyapplied for routine analysis in research institutions, quality control department in meant in industries, approvedtesting laboratories studies in near future.Key Words: Bilastine, Montelukast, LC method

A simple and selective LC method is described for the determination of Sildenafil and dapoxetinein tablet dosageforms. Chromatographic separation was achieved on a c18 column using mobile phase consisting of a mixture of 30volumes of ammonium acetate buffer,40 volumes of acetonitrile and 30 volumes of Methanol with detection of 239nm. Linearity was observed in the range 50-175 μg/ml for Sildenafil (r2 =0.998) and 50-175 μg /ml for dapoxetine(r2=0.999) for the amount of drugs estimated by the proposed methods was in good agreement with the label claim. Theproposed methods were validated. The accuracy of the methods was assessed by recovery studies at three differentlevels. Recovery experiments indicated the absence of interference from commonly encountered pharmaceuticaladditives. The method was found to be precise as indicated by the repeatability analysis, showing %RSD less than 2.All statistical data proves validity of the methods and can be used for routine analysis of pharmaceutical dosageform.Key Words: Sildenafil, dapoxetine, LC method

The present study has been a satisfactory attempt to formulate a floating Microspheres double walled antifungal ofPosaconazole with a view to control the release of the drug. From the experimental results it can be concludedthat, FT-IRstudyshowsnosignificantshiftingofthepeaksthereforeitconfirms the short term stability of the drug in themicrospheres. Biocompatible polymers like can be HPMC, Ethyl cellulose and Eudragitused to formulate a floatingMicrospheres .Good percentage drug entrapment and practical yields were obtained with the polymers.Theflowpropertiesofallformulationswerewithintheacceptablerangeand therefore they could be easily filled intocapsules. The floating microspheres of drug with HPMC and Ethyl cellulose were buoyant while those withEudragit S 100 showed greater buoyancy. Cumulativepercentagedrugreleasesignificantlydecreasedwithincreaseinpolymer concentration. Formulated microspheres were stable and compatible at the room and acceleratedtemperature and humidity in storage for 90days. Thus, the formulated floating microspheres seem to be a potentialcandidate as an oral gastroprotective controlled drug delivery system in prolonging the drug retention stomach andincreasing the bioavailability of drug. .Formulated microspheres were stable and compatible at the room andaccelerated temperature and humidity in storage for 90days. Thus, the formulated floating microspheres seem to bea potential candidate as an oral gastroprotective controlled drug delivery system in prolonging the drug retentionstomach and increasing the bioavailability of drug.Key Words: Posaconazole, floating microspheres

The present research work involves formulation and invitro evaluation of Miconazole transfersomal gel to reducedosing frequency. The FTIR spectra revealed that there was no interaction between the drug and excipients.Transfersome formulations were prepared by hand shaking modified thin film hydration technique and wereincorporated into 1.5% carbopol gel. The Formulation Mf7 containing Lecithin: Tween-80 has higher entrapmentefficiency and maximum drug release. Stability studies performed for optimized transfersome gel formulationsindicates that prepared transfersomes have more stability at lower temperature. Based on the above data, it wasconfirmed that prepared Miconazole, transfersomal gels can be considered as one of the promising approach toreduce the dosing frequency and to maintain drug concentration at the desired site for longer time.Key Words: Miconazole, transfersomal gel

Cubosomes are self assembled liquid crystalline particles that are unique, sub-micron nano structured particlesmainly prepared using amphiphilic lipids and an appropriate stabilizer.Amphiphilic lipids can spontaneously self-assemble with proper ratio of water to form a cubic phase. Cubic phases can enclose hydrophilic, hydrophobic andamphiphilic drugs for delivery. Nanostructured cubosomes, prepared by fragmentation of bulk cubic phase gels orlyotropic methods that retain the same inner structure of cubic phase.Their lipid bilayers are arranged in 3D spacesuch that they have an uninterruptedcubicsurface, separated by two interconnected aqueous channels. Thus, theyhave a large surfacearea and lower viscosity involving numerous internal segments, giving them a definitiveadvantage over other lamellar vesicles infacilitating the better entrapment efficiency and also sustained release ofactive therapeutic substances.These unique properties make cubosomes excellent novel delivery systems applicablefor oral, mucosal, transdermal, parenteral and even ocular drug delivery.The major limitation for transdermalapplication is the permeability issue since the drug has to permeate through a major skin barrier, Stratumcorneum.The excellent penetrationenhancing property of cubosomes made them use much in delivery of drugsthrough transdermal route.Their self-assemblingproperties make their production uncomplicated, with two majormanufacturing techniques: the top-downand bottom-up techniques.Cubosomes are having wide range ofapplications in various fields and they can be characterized by various evaluation parameters. So, Cubosomesthatare gaining more attention in pharmaceutical field are discussed in this review.Key Words: Cubosomes,nanostructure,cubic phase, transdermal